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Thermo Scientific™ Kit mini de purificación de ADN genómico de sangre completa GeneJET™
Perform silica membrane-based genomic DNA purification from fresh, frozen anticoagulated mammalian blood, aged blood, buffy coat, bone marrow and body fluids.
Marca: Thermo Scientific™ K0781
Detalles adicionales : Peso : 0.58186kg
Descripción
The kit utilizes silica based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions or time consuming alcohol precipitation.The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30kb in size.Efficient: High yields of high molecular weight genomic DNA
Fast: 20 minute procedure following cell lysis
Convenient: Spin columns are capped and assembled with collection tubes
Pure: DNA free of enzyme inhibitors, RNA and proteins, ready to use for all demanding applications, validated in realtime PCR, endpoint PCR, multiplex PCR and restriction enzyme digests
Compatible: Suitable for wide variety of samples, like fresh or frozen anticoagulated mammalian blood (treated with EDTA, Citrate or Heparine), aged blood, buffy coat, bone marrow, body fluids
Includes:
Proteinase K Solution, Lysis Solution, Wash Buffer WB I (concentrated), Wash Buffer II (concentrated), Elution Buffer, GeneJET™ Purification Columns (pre-assembled with collection tubes), Collection tubes, Detailed Protocol
Recommended for:
Isolated genomic DNA is ideal for use in common molecular biology procedures, including PCR and qPCR, FastDigest™ and conventional restriction digestion and Southern blotting.
Especificaciones
| El ADN genómico aislado es apto para su uso en procedimientos habituales de biología molecular, tales como: PCR y qPCR, digestión FastDigest y convencional por restricción, Southern Blot. | |
| Solución de proteinasa K, solución de lisis, tampón de lavado WB I (concentrado), tampón de lavado II (concentrado), tampón de elución, columnas de purificación GeneJET™ (preacopladas a los tubos de recogida de muestras), tubos de recogida de muestras, protocolo detallado | |
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| Kit mini de purificación de ADN genómico de sangre completa Thermo Scientific™ GeneJET™ |