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Thermo Scientific™ Kit de mesopreparación de plásmidos GeneJET™

Perform large-scale isolation of high-quality plasmid DNA from recombinant E. coli cultures with this kit.

Marca:  Thermo Scientific™ K0481

Detalles adicionales : Peso : 4.10950kg

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Código de producto. 11531645

  • 152.00€ / cada uno


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Descripción

Descripción

The kit utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 200μg of high copy number plasmid DNA. The purified plasmid DNA can be used in a wide variety of molecular biology procedures such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing, in vitro transcription and transfection of robust cell lines.

  • Efficient: High yields up to 200μg of high quality plasmid DNA from 50mL of bacterial culture

  • High concentration of DNA purified: 400ng/μl

  • Convenient: Spin column format, centrifuge or vacuum protocols

  • Fast: Procedure takes 60min, considerably faster than using gravity flow columns

Includes:

Resuspension Solution, Lysis Solution, Neutralization Solution, Endotoxin Binding Reagent, Wash Solution I (concentrated), Wash Solution II (concentrated), RNase A Solution, Elution Buffer (10mM Tris-HCl, pH 8.5), GeneJET™ Midi Purification Columns pre-assembled with collection tubes (15mL), Collection tubes (15mL)

Recommended for:

FastDigest™ restriction enzyme digestion, automated fluorescent sequencing, PCR, cloning, transformation, in vitro transcription, transfection of robust cell lines.

Especificaciones

Especificaciones

400 ng/µl
Solución de resuspensión, solución de lisis, solución de neutralización, reactivo de unión de endotoxina, solución de lavado I (concentrado), solución de lavado II (concentrado), solución ARNasa A, tampón de elución (10 mM de Tris-HCl, pH 8.5), columnas de purificación premontadas GeneJET™ Midi
Kit de mesopreparación de plásmidos GeneJET™
Digestión de enzimas de restricción FastDigest®, secuenciación fluorescente automatizada, PCR, clonación, transformación, transcripción in vitro, transfección de líneas celulares resistentes.
25 preparaciones
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