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Thermo Scientific™ Bpu1102I (BlpI)

Cut at GC^TNAGC sites with Bpu1102I (BlpI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: BlpI, Bsp1720I, CelII).

Marca:  Thermo Scientific™ ER0092

Detalles adicionales : Peso : 0.00900kg

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Código de producto. 10450460

  • 132.00€ / 1000 unidades

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3'...C G A N T▵C G...5'

Conditions for 100% Activity:

  • 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
  • Incubate at 37°C

Storage Buffer:

  • Bpu1102I is supplied in:10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Ligation and Recleavage:

  • After 50-fold overdigestion with Bpu1102I, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20-40 u of T4 DNA Ligase/1μg of fragments and 10% PEG. More than 95% of these can be recut

Methylation Effects:

  • Dam: never overlaps — no effect
  • Dcm: never overlaps — no effect
  • CpG: may overlap — no effect.
  • EcoKI: never overlaps — no effect.
  • EcoBI: may overlap — effect not determined

Digestion of Agarose-embedded DNA:

  • Minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda DNA in 16hours
  • Compatible Ends: Bpu10I, Eco81I, HpyF3I