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F4/80 Monoclonal Antibody (BM8), Super Bright™ 645, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

en la oferta

Especificaciones

Antígeno F4/80
Clon BM8
Concentración 0.2 mg/mL
Aplicaciones Flow Cytometry
Clasificación Monoclonal
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Descripción

Descripción

Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin. Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis of mouse splenocytes. Use isotype control at the same concentration as experimental antibody. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

F4/80 Monoclonal antibody specifically detects F4/80 in Mouse samples. It is validated for Flow Cytometry.
Especificaciones

Especificaciones

F4/80
0.2 mg/mL
Monoclonal
Liquid
RUO
Q61549
IgG2a κ
Affinity chromatography
Antibody
BM8
Flow Cytometry
Super Bright 645
Rat
Mouse
13733
Primary
4° C, store in dark, DO NOT FREEZE!
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