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Gibco™ DMEM/F-12, powder

DMEM/F-12, powder

Marca:  Gibco™ 32500043

Detalles adicionales : Peso : 0.60000kg

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Código de producto. 11504466

  • 167.00€ / 50 litros


Fecha de envío estimada
del proveedor 01-10-2018
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Descripción

Descripción

Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool. Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco™ DMEM / F-12 is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco™ DMEM / F-12 product made in our Grand Island facility (12500-096). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Especificaciones

Especificaciones

ISO 13485 standards, FDA
Mammalian cell culture
With additives L-glutamine, phenol red, without additives HEPES, sodium bicarbonate
6.2 to 6.6
50L
2 to 8°C, room temperature
HEPES, sodium bicarbonate
DMEM/F-12, 50L, Powder, 6.2 to 6.6 pH, 250 to 290mOsm/kg Osmolality, 36 Months Shelf Life, Ambient Shipping Condition, 2 to 8°C Storage Requirements, Animal Origin-Free Classification, For Mammalian Cell Culture
Powder
1 Each
DMEM/F-12
CE-IVD
L-glutamine, phenol red, sodium pyruvate
2 to 8°C
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