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Molecular Probes™ Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI)

Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide (PI)

Marca:  Molecular Probes™ V13245

Detalles adicionales : Peso : 0.00001kg

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Código de producto. 10652071

  • 520.00€ / cada uno


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Descripción

Descripción

The Dead Cell Apoptosis Kit with Annexin V Alexa Fluor™ 488 & Propidium Iodide is a flow cytometry kit used to measure early apoptosis by detecting phosphatidyl serine expression and membrane permeability.

View a selection guide for all apoptosis assays for flow cytometry.

Superior brightness
Unlike other Annexin V kits that have lower protein concentrations or purification levels, the Annexin V Alexa Fluor™ 488 conjugate is optimized for flow cytometry to provide the largest separation between apoptotic and live cells. The Alexa Fluor™ 488 dye is a superior green-fluorescent dye with a spectrum similar to fluorescein (FITC).

High binding efficiency
Annexin V conjugates are made from a highly purified cys-annexin, which leads to higher binding efficiency, resulting in highly accurate characterization of the apoptotic process.

Multi-parametric
Many publications require a minimum of two different ways to identify that cells are apoptotic. This multi-parametric kit detects phosphatidyl serine (PS) on the cytoplasmic surface of the cell membrane and membrane integrity using propidium iodide.

How it works
When cells are stained with Annexin V and propidium iodide, apoptotic cells expressing PS show green fluorescence, which can be detected in the FITC channel, and low red fluorescence. Dead or necrotic cells show bright red fluorescence and no green fluorescence, while live cells show no green or red fluorescence.
Especificación

Especificación

Dead Cell Apoptosis Kit, 2 250 rxns, Slide/Tube Format, 521nm Emission (Alexa Fluor™ 488), 499nm Excitation (Alexa Fluor™ 488), 617nm Emission (PI), 535nm Excitation (PI), Wet Ice Shipping Condition, 2 to 8°C Storage Temperature, Water Soluble
Dead Cell Apoptosis Kit
Fluorescent
Cell viability, proliferation and function, flow cytometry
2 to 8°C
Dead Cell Apoptosis Kit
250 Assay(s)
Slide, Tube
Alexa Fluor™ 488, propidium iodide
5 vials of annexin V, Alexa Fluor™ 488 conjugate (250μL per vial), 1 Vial of propidium iodide (PI, 100μL), and 1 bottle of annexin binding buffer (5X solution, 50mL)
250
Videos

For Research Use Only. Not for use in diagnostic procedures.