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STEMCELL Technologies CoV Protease Substrate-1 TF5
FRET-based peptide substrate for detection of protease activity of coronaviruses
Marca: STEMCELL Technologies 100-0505
Detalles adicionales : Peso : 0.00110kg
Descripción
- CoV Protease Substrate-1 TF5 allows for efficient detection of coronavirus (CoV) proteases
- This fluorescence resonance energy transfer (FRET)-based peptide substrate is cleaved by 3C-like proteases (3CLpro), the CoV main protease involved in regulating the coronavirus replication complex
- 3CLpro is also a potential target for therapeutic development
- CoV Protease Substrate-1 TF5 has an excitation wavelength of 649 nm, an emission wavelength of 664 nm, and an extinction coefficient of 250,000 cm-1 M-1
- It consists of a 14 amino acid sequence (KTSAVLQSGFRKME) that also contains the Tide Quencher™ 5 (TQ5; quencher) and Tide Fluor™ 5 (TF5; donor) fragments at the N- and C- termini, respectively
- When the peptide substrate is intact, favorable energetic overlap of the TF5 excited state and the TQ5 absorption leads to efficient intramolecular fluorescence quenching of TF5 by the TQ5 fragment
- Cleavage of the substrate by CoV proteases, however, dissociates the TF5 fragment from TQ5, restoring the fluorescence of the TF5 moiety that is directly correlated to the CoV protease activity
Especificaciones
Enzyme Substrate; FRET | |
100 Tests |