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Thermo Scientific™ CellSensor™ AP-1-bla HEK293T Cell Line

Product Code. 10062913
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10062913 1 Vial Each
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Product Code. 10062913 Supplier Thermo Scientific™ Supplier No. K1658

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Description

The CellSensor™ AP-1-bla HEK 293T Cell Line contains a beta-lactamase reporter gene under control of the AP-1 response element stably integrated into HEK 293T cells.

The CellSensor™ AP-1-bla HEK 293T Cell Line contains a beta-lactamase reporter gene under control of the AP-1 response element stably integrated into HEK 293T cells. The cell line was created through sorting by FACS of cells responsive to stimulation of the AP-1 pathway with phorbol 12-myristate 13-acetate (PMA). The cell line was validated for DMSO tolerance, incubation time with stimulant, and substrate loading conditions. The AP-1-bla HEK 293T cell line responds to agonist treatment as expected from literature and can be adapted for high throughput screening for agonists or antagonists of the AP-1 pathway with compound libraries. Candidate drugs can also be tested for dose response against this cell line. Academic and non-profit customers, please inquire for special pricing.

TRUSTED_SUSTAINABILITY

Spécification

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage CellSensor™ Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
Description CellSensor™ AP-1-bla HEK293T Cell Line, 1Vial, ∽2,000,000 cells/vial at 2,000,000 cells/mL Quantity of Cells, AP-1-bla HEK293T Cell Line, Fluorescent Detection Method, CellSensor™ Druggable Target, Adherent Growth Properties, Liquid Nitrogen Storage
Detection Method Fluorescent
For Use With (Application) Pharma and biopharma, drug discovery and development, target and lead identification and validation, pathway biology, cellular pathway analysis assays, cellsensor™ cellular pathway assays
Includes beta-lactamase reporter gene under control of the AP-1 response element stably integrated into HEK 293T cells
Product Line CellSensor
Quantity 1 Vial
Sample Type AP-1-bla HEK293T
Target Entry EGFR, ERK1/2, p38, MEK, MAPK pathway, c-MET, JNK, RON, RAF
Type Cell Line
System Type CellSensor™
Druggable Target Signaling Pathway
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For Research Use Only. Not for use in diagnostic procedures.

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