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CD80 (B7-1) Monoclonal Antibody (2D10.4), Super Bright™ 436, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
en la oferta
Especificaciones
Antígeno | CD80 (B7-1) |
---|---|
Clon | 2D10.4 |
Concentración | 5 μL/Test |
Aplicaciones | Flow Cytometry |
Clasificación | Monoclonal |
Descripción
Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily. CD80 is expressed by activated B cells, macrophages and dendritic cells. In addition, activated T cells express this antigen. CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4). The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively. Applications Reported: This 2D10.4 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of Daudi cell line. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
CD80 (B7-1) Monoclonal antibody specifically detects CD80 (B7-1) in Human samples. It is validated for Flow Cytometry.Especificaciones
CD80 (B7-1) | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
P33681 | |
CD80 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
CD80 |
2D10.4 | |
Flow Cytometry | |
Super Bright 436 | |
Mouse | |
Human | |
941 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |