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CD48 Armenian Hamster anti-Mouse, Super Bright 600, Clone: HM48-1, eBioscience™

Armenian Hamster Monoclonal Antibody

Marca:  Invitrogen 63-0481-82

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15923514

  • 279.00€ / 100 microgramos


Fecha de envío estimada
del proveedor 01-07-2019
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Descripción

Descripción

Applications Tested: This HM48-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (cat. SB-4400) to minimize any non-specific polymer interactions. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily that includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of the Ig family, thus mediating homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Further, signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases. CD48 is strongly expressed on lymphocytes and monocytes, weakly on granulocytes but is absent on platelets, fibroblasts, epithelium and endothelium. CD48 is one of marker for detecting the defects of GPI anchoring structure on the patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. 2B4-CD48 interaction may be involved in the pathology of allergic airway diseases and provide a pharmacological target for the treatment of diseases such as allergic rhinitis and asthma.
Especificaciones

Especificaciones

CD48
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P18181
BCM1, BLAST, BLAST1, MEM-102, SLAMF2, hCD48, mCD48, CD48d
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
Flow Cytometry
HM48-1
Super Bright 600
Cd48
Liquid
Cd48
IgG
100 μg
4° C, store in dark, DO NOT FREEZE!
Primary
12506
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