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CD45R (B220) Rat anti-Mouse, Super Bright 780, Clone: RA3-6B2, eBioscience™

Rat Monoclonal Antibody

Marca:  Invitrogen 78-0452-80

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15934154

  • 141.00€ / 25 microgramos


Fecha de envío estimada
del proveedor 01-10-2019
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Descripción

Descripción

Please refer to the datasheet for Super Bright Staining Buffer for more information. In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product No. 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product No. A10497). Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser CD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.
Especificaciones

Especificaciones

CD45R (B220)
Monoclonal
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
P08575
PTPRC
IgG2a, kappa
25 μg
4° C, store in dark, DO NOT FREEZE!
Primary
19264, 5788
Flow Cytometry
RA3-6B2
Super Bright 780
PTPRC
Liquid
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Mouse
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