CD31 (PECAM-1) Monoclonal Antibody (WM-59 (WM59)), Super Bright™ 436, eBioscience™, Invitrogen™

Descripción

Description: The WM59 monoclonal antibody reacts with human CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction. Applications Reported: This WM/59 antibody has been reported for use in flow cytometric analysis. Applications Tested: This WM/59 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at less than or equal to 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions.

CD31 (PECAM-1) Monoclonal antibody specifically detects CD31 (PECAM-1) in Human samples. It is validated for Flow Cytometry.