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CD278 (ICOS) Mouse anti-Human, Super Bright 645, Clone: ISA-3, eBioscience™

Mouse Monoclonal Antibody

Marca:  Invitrogen 64-9948-42

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15933684

  • 301.00€ / paquete de 100


Fecha de envío estimada
del proveedor 27-11-2019
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Descripción

Descripción

Applications Tested: This ISA-3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product No. SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 645 nm; Laser: Violet Laser SuperBright Polymer Dyes are sold under license from Becton, Dickinson and Company. CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.
Especificaciones

Especificaciones

CD278 (ICOS)
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
Q9Y6W8
ICOS
IgG1, kappa
100 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
29851
Flow Cytometry
ISA-3
Super Bright 645
ICOS
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
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