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CD25 Mouse anti-Human, Super Bright 780, Clone: BC96, eBioscience™

Mouse Monoclonal Antibody

Marca:  Invitrogen 78-0259-41

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15934144

  • 150.00€ / paquete de 25


Fecha de envío estimada
del proveedor 27-09-2019
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Descripción

Descripción

Please refer to the datasheet for Super Bright Staining Buffer for more information. In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product No. 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product No. A10497). Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser CD25 (IL2 receptor alpha chain/IL2RA) is a cytokine that plays a role in the proliferation of T and B lymphocytes. The receptor of this cytokine (IL2RA) is a heterotrimeric protein complex with a gamma chain also shared by interleukin 4 (IL4) and interleukin 7 (IL7). IL2RA, IL2R beta chain (IL2RB), and the IL2R gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric IL2RA chains result in low-affinity receptor, while homodimeric IL2RB chains produce a medium-affinity receptor. The expression of IL2 in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. IL2 is primarily produced by mature T cells. IL2 plays an important role as a growth factor, differentiation factor, and regulator of cell death. IL-2 stimulates the proliferation of B cells, augments natural killer cell activity, and inhibits granulocyte macrophage colony formation. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests a role in the immune response to antigenic stimuli. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.
Especificaciones

Especificaciones

CD25
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P01589
CD25; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL-2Ra; interleukin 2 receptor, alpha; Interleukin-2 Receptor alpha; Interleukin-2 receptor subunit alpha; p55; TAC antigen
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
BC96
Super Bright 780
IL2RA
Liquid
IL2RA
IgG1, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
3559
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