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CD20 Mouse anti-Human, Super Bright 780, Clone: 2H7, eBioscience™

Mouse Monoclonal Antibody

Marca:  Invitrogen 78-0209-41

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15914144

  • 135.00€ / paquete de 25


Fecha de envío estimada
del proveedor 02-07-2019
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Descripción

Descripción

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product No. 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product No. A10497). Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most maligt B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell maligcies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
Especificaciones

Especificaciones

CD20
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P11836
B1, Bp35, CD20, LEU-16, MGC3969, MS4A1
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
2H7
Super Bright 780
MS4A1
Liquid
MS4A1
IgG2b, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
931
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