CD2 Monoclonal Antibody (RPA-2.10), Super Bright™ 645, eBioscience™, Invitrogen™

Descripción

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells. Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm. We recommend using a 660/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

CD2 Monoclonal antibody specifically detects CD2 in Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human Primate, Porcine, Rhesus Monkey samples. It is validated for Flow Cytometry.