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CD16 Mouse anti-Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human primate, Rhesus Monkey, Super Bright 600, Clone: 3G8, eBioscience™

Mouse Monoclonal Antibody

Marca:  Invitrogen 63-0166-41

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15933474

  • 141.00€ / paquete de 25


Fecha de envío estimada
del proveedor 27-09-2019
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Descripción

Descripción

Applications Reported: This 3G8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 3G8 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should bedetermined empirically. Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
Especificaciones

Especificaciones

CD16
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
O75015, P08637
FCGR3A, FCGR3B
IgG1
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
2214, 2215
Flow Cytometry
3G8
Super Bright 600
FCGR3B
Liquid
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Baboon, Chimpanzee, Cynomolgus Monkey, Human, Non-human primate, Rhesus Monkey
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