Invitrogen™ IL-8 Canine ELISA Kit

Descripción

The Canine Interleukin 8 (Dog IL-8) ELISA quantitates Dog IL-8 in canine serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Dog IL-8. Principle of the method The Canine IL-8 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

IL-8 (interleucina-8) es una quimiocina CXC pro-inflamatoria de 72 aminoácidos designada CXCL8, secretada por células endoteliales y monocitos, que actúa como un importante quimioatrayente para los neutrófilos y también las células T y los basófilos. IL-8, aislada por primera vez de células de osteosarcoma, contiene el motivo ELR (secuencia N-terminal de aminoácidos Glu-Leu-Arg) y señala a través de los receptores CXCR1 y CXCR2. Los estudios de cáncer han demostrado el papel de IL-8 en la angiogénesis y el crecimiento de los tumores. La nomenclatura anterior para IL-8 incluye la proteína activadora de neutrófilos 1 (NAP-1), la proteína quimiotáctica de granulocitos 1 (GCP-1), el péptido activador de neutrófilos derivado de monocitos (MONAP) y la proteína 3-10C.