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Armenian Hamster IgG, Super Bright 780, eBioscience™

Armenian Hamster Isotype Control

Marca:  Invitrogen 78-4888-80

Detalles adicionales : Peso : 0.01000kg

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Código de producto. 15994174

  • 136.00€ / 25 microgramos
Envío estimado: 08-02-2022

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Descripción

Descripción

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100μL of cell sample + 100μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 405nm; Emission: 780nm; Laser: Violet Laser

The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

Especificaciones

Especificaciones

Armenian Hamster IgG
Control
0.2 mg/mL
PBS with BSA and 0.09% sodium azide; pH 7.2
IgG
IgG
25 μg
4°C, store in dark, DO NOT FREEZE!
Not Applicable
Control, Flow Cytometry
eBio299Arm
Super Bright 780
Liquid
Hamster
Affinity chromatography
RUO
Isotype Control
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Ofertas especiales