Leukemia Inhibitory Factor Mouse anti-Human, PE, Clone: 1F10, BD

Descripción

The 1F10 antibody specifically binds to human Leukemia Inhibitory Factor (LIF) also known as Human Interleukin for DA cells (HILDA), Melanoma-derived Lipoprotein Lipase Inhibitor (MLPLI) and Hepatocyte Stimulating Factor III (HSF III). LIF is produced by multiple sources including activated T cells and macrophages, myelomonocytic lineages, fibroblasts, liver, heart and melanoma cells. LIF regulates the differentiation of embryonic stem cells, neural cells, osteoblasts, adipocytes, hepatocytes and kidney epithelial cells. Other activities include terminal differentiation in leukemic cells and the stimulation of acute-phase protein synthesis in hepatocytes. Many of its biological functions parallel those of Interleukin-6, Oncostatin M, Ciliary Neurotrophic Factor, Interleukin-11 and Cardiotrophin-1. In vivo LIF is important in regulating the inflammatory response by tuning the balance of four systems in the body, namely the immune, the haematopoietic, the nervous and the endocrine systems. The immunogen used to generate the 1F10 hybridoma was recombinant vaccinia virus encoding the human LIF cytokine. The LIF cytokine had been engineered to get expressed on the membrane of the infected cell. The attachment to the membrane was obtained with the glycosylphoshosphatidyl anchor targeting DNA sequence from the DAF molecule (CD55). This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

Flow Cytometry