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KDEL Polyclonal Antibody, Invitrogen™
Rabbit Polyclonal Antibody
Marca: Invitrogen PA1013
Detalles adicionales : Peso : 0.01000kg
Descripción
PA1-013 detects KDEL from human, rat, mouse and hamster. PA1-013 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 57 kDa protein representing protein disulfide isomerase (PDI), a 78 kDa protein representing glucose regulated protein 70 kDa (GRP78) and an ∽94 kDa protein representing GRP94 in mouse myeloma cells. Immunofluorescence staining of GRP78 in mouse myeloma cells with PA1-013 results in diffuse cytoplasmic staining consistent with specific ER localization. PA1-013 immunoprecipitates PDI, GRP78, an ∽72 kDa protein which may be endoplasmic reticulum protein 72 kDa (ERp72), and an ∽94 kDa protein representing GRP94 from numerous rodent tissues. The PA1-013 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94. Reconstitute in 100 μL PBS to create a stock of 1 mg/mL.
KDEL Polyclonal antibody specifically detects KDEL in Hamster, Human, Mouse, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot.Abbreviations: AP=Affinity Purification, ChIP=Chromatin IP, CM=Confocal Microscopy, DB=Dot Blot, EIA=ELISA, EM=Electron Microscopy, FC=Flow Cytometry, GS=Gel Supershift, IC=Immunocytochemistry, IF=Immunofluorescence, IH=Immunohistology/Immunohistochemistry, IP=Immunoprecipitation, IPK=IP Kinase Assay, KA=Kinase Assay, NEUT=Neutralization, PIA=Peptide Inhibition Assay, RIA=Radioimmunossay, TCA=T Cell Activation, WB=Western Blotting, Species: Am=Amphibian, Av=Avian, B=Bovine, Ca=Cat ,Ck=Chicken, D=Dog, Dr=Deer, Ds=Drosophila, El=Elk, Fg=Frog, Fs=Fish, Ft=Ferret, G=Goat, Gp=Guinea Pig, H=Horse, Hm=Hamster, Hu=Human, K=Kangaroo, Ms=Mouse, Nhp=Non-Human Primate, O=Ovine, P=Porcine, Rb=Rabbit, Rp=Reptile, Rt=Rat, Ys=Yeast, Xn=Xenopus, (wk)=cross reacts weakly
Especificaciones
KDEL | |
Polyclonal | |
Unconjugated | |
Kdelr1 | |
78 kDa glucose-regulated protein; 8030486F04Rik; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250; AL022860; AU019543; AW215843; baffled; Binding-immunoglobulin protein; BiP; D2Wsu141e; D2Wsu17e; ECGP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; endoplasmic reticulum resident protein 58; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250; endothelial cell (HBMEC) glycoprotein; EP58; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ER protein 58; ERD2; Erd2.1; ERp58; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein; glucose-regulated protein, 78kDa; gp96; gp96 homolog; GRP 78; Grp78; GRP-78; GRP94; GRP-94; HDEL; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (gl | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
100689305, 100750912, 100773331, 10945, 14828, 22027, 25617, 3309, 361577, 362862, 68137, 7184 | |
-20° C, Avoid Freeze/Thaw Cycles | |
Lyophilized |
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot | |
1 mg/mL | |
antibody with no preservative | |
P06761, P08113, P11021, P14625, P20029, P24390, Q569A6, Q66HD0, Q99JH8 | |
Hsp90b1, HSPA5, Kdelr1 | |
Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94. | |
100 μg | |
Primary | |
Hamster, Human, Mouse, Rat | |
Antibody | |
IgG |