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Clon (DYKDDDDK) de etiqueta de epítopo anti-FLAG™: FG4R

Mouse Monoclonal Antibody

Marca:  Invitrogen MA191878

Detalles adicionales : Peso : 1.24740kg

Código de producto. 11525702

  • 187.00€ / 100 microgramos


Stock EU 12
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Descripción

Descripción

DYKDDDDK Tag (FLAG-tag, FLAG octapeptide) is a polypeptide protein tag that can be added to a protein using recombit DNA technology. The FLAG tag structure has been optimized for compatibility with the proteins it is attached to, in that it is more hydrophilic than other common epitope tags and therefore less likely to denature or inactivate proteins to which it is appended. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. Tagging with xxxDDDDK may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, and in internal positions of the target protein. The small size of the epitope tag and its high hydrophilicity tend to decrease the possibility of interference with protein expression, proteolytic maturation, antigenicity and function. The enterokinase cleavage site allows it to be completely removed from the purified fusion proteins.
Especificaciones

Especificaciones

Etiqueta de epítopo DYKDDDDK (FLAG«)
Monoclonal
1 mg/mL
100 µg de anticuerpo (1 mg/ml) en PBS (pH 7.2) con glicerol al 50 %.
DDDDK; DDDDK epitope tag; DYKDDDDK epitope tag; ECS epitope tag; ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; FLAG; FLAG Tag
Péptido sintético (DYKDDDDK) acoplado a KLH.
Protein A
RUO
Principal
Monoclonal
Inmunotransferencia (Western Blotting), Inmunotransferencia (Western Blotting), Inmunotransferencia (Western Blotting), Inmunotransferencia (Western Blotting)
FG4R
Sin marcar
Sin purificar
Ratón
IgG2b
100 µg
Almacenar a 4 °C a corto plazo. Para el almacenamiento a largo plazo, almacenar a - 20 °C. Evitar los ciclos de congelación y descongelación.
Primary
Tag
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