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Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents with manual

Marca:  Applied Biosystems™ 4387391M

Detalles adicionales : Peso : 0.00450kg

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Código de producto. 10794207

  • 1502.00€ / cada uno


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Descripción

Descripción

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
  • Recommended for RNA pathogen amplification
  • Consistent amplification of RNA targets with high specificity and sensitivity
  • Optimized to work with your target-specific primers and probes
  • Contains ROX for quantitative fluorescent signal normalization

    AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization. A Detection Enhancer is also provided as an optional reagent for amplification of templates with a high GC content or persistent secondary structure.
  • Especificaciones

    Especificaciones

    2X RT-PCR
    Primer-probe detection
    AgPath-ID™ One-Step RT-PCR Reagents with Manual
    7500 system
    1000 rxn(s)
    TaqMan™
    1000 rxn(s)
    AgPath-ID™ One-Step RT-PCR Reagents with Manual, 1000 rxns, ROX (Pre-mixed) Dye, TaqMan™ Primer-Probe Compatibility, Primer-Probe Detection Method, AmpliTaq Gold™ DNA Polymerase, ArrayScript™ Reverse Transcriptase, Standard Reaction Speed, Used for Animal Health and Real Time PCR (qPCR)
    ROX (Pre-mixed)
    Animal health, real time PCR (qPCR)
    14mL 2X RT-PCR buffer, 1100μL 25X RT-PCR enzyme mix, 2 x 1.2mL detection enhancer, 25mL nuclease-free water
    TaqMan™
    AgPath-ID™ One-Step RT-PCR Reagents with Manual
    1 hr.
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    For Research Use Only. Not for use in diagnostic procedures.