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Beschreibung
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
- Recommended for RNA pathogen amplification
- Consistent amplification of RNA targets with high specificity and sensitivity
- Optimized to work with your target-specific primers and probes
- Contains ROX for quantitative fluorescent signal normalization
AgPath-ID™ One-Step RT-PCR Reagents are configured for fast and simple reaction setup. The reactions are assembled in a single tube, minimizing sample handling errors and expediting set-up time. Once assembled, results are available in approximately one hour. The 25X RT-PCR Enzyme Mix included in the kit contains highly efficient ArrayScript™ Reverse Transcriptase, a mutant MMLV RT that produces high cDNA yields, and AmpliTaq Gold™ polymerase, the preferred hot-start DNA polymerase for specific target amplification. The 2X RT-PCR Buffer has been optimized for efficient, robust reverse transcription and PCR includes the passive reference dye, ROX™ Dye, for quantitative fluorescent signal normalization.
Spezifikation
Spezifikation
| Concentration | 25X |
| Content And Storage | Sufficient for 100 reactions of 25 μL, contains: • 1375μL 2X RT-PCR Buffer • 110μL 25X RT-PCR Enzyme Mix • 1.75mL Nuclease-free Water. |
| Description | Works on all AB platforms, but is optimized for the 7500. |
| Detection Method | Primer-probe |
| Format | Tube |
| GC-Rich PCR Performance | High |
| PCR Method | 1-step RT-qPCR |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Reaction Speed | Standard |
| For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex, QuantStudio™ Absolute Q Digital PCR System |
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For Research Use Only.
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