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OPTIZYME™ T4 DNA Polymerase, Fisher BioReagents™
133.00€ - 153.04€
Especificaciones
Concentración | 5 U/μL |
---|---|
Color | Colorless |
pH | 7.5 |
Para utilizar con (aplicación) | Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase |
Tampón de almacenamiento | OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM |
Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
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Código de producto | Marca | Cantidad | Precio | Cantidad y disponibilidad | |||||
12847162
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Fisher Bioreagents
BP8105-1 |
100 U |
133.00€
100 unidades |
Fecha estimada de envío: 14-05-2024 Inicie sesión para ver el stock |
Por favor, inicie sesión para comprar este producto. ¿No tiene usuario web? Regístrese hoy mismo. | ||||
12857162
|
Fisher Bioreagents
BP8105-5 |
500 U |
Este artículo actualmente no está disponible o ha sido discontinuado. Ver la página del producto para posibles alternativas |
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Descripción
OPTIZYME™ T4 DNA Pol is a template-dependent DNA polymerase that catalyzes 5'->3' synthesis from primed single-stranded DNA.
Applications and features:
- OPTIZYME T4 DNA Polymerase creates blunt DNA ends by filling in 5'-overhangs and/or removing 3'-overhangs
- Creation of blunt PCR products with 3'-dA overhangs
- Oligonucleotide-directed site-specific mutagenesis
- Synthesis of labeled DNA probes (by replacement reaction)
- Ligation-independent cloning of PCR products
- Active in buffers for OPTIZYME restriction enzymes, reverse transcriptases and T4 DNA ligase
- 3'->5' exonuclease activity
- Lacks 5'->3' exonuclease activity
Supplied with:
5X OPTIZYME T4 DNA Pol Buffer: 335mM Tris-HCl (pH 8.8 at 25°C), 33mM MgCl2, 5mM DTT, 84mM (NH4)2SO4
Source: E. Coli cells with a cloned gene 43 of bacteriophage T4
Quality Control:
Endodeoxyribonuclease-free
Especificaciones
5 U/μL | |
7.5 | |
OPTIZYME™ T4 DNA Pol Buffer 5X: Tris HCl 335mM (8.8 pH at 25°C), MgCl2 33mM, DTT 5mM, (NH4)2SO4 84mM | |
E. coli cells with a cloned gene 43 of bacteriophage T4 | |
Liquid |
Colorless | |
Synthesis of labeled DNA probes, creation of blunt PCR products with 3'-dA overhangs, ligation-independent cloning of PCR products, Restriction Enzymes, reverse transcriptases and T4 DNA ligase | |
-20°C | |
T4 DNA Polymerase |