Invitrogen™ TNFRSF11B (OPG) Human ELISA Kit

Descripción

The Human TNFRSF11B (OPG) ELISA quantitates Hu OPG in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu OPG. Principle of the method The Human OPG solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Actúa como receptor de señuelo para TNFSF11/RANKL y, por lo tanto, neutraliza su función en la osteoclastogénesis. Inhibe la activación de los osteoclastos y promueve la apoptosis de los osteoclastos in vitro. La homeostasis ósea parece depender de la proporción local entre el TNFSF11 y el TNFRSF11B. También puede jugar un papel en la prevención de la calcificación arterial. Puede actuar como receptor señuelo para TNFSF10/TRAIL y proteger contra la apoptosis. La unión TNFSF10/TRAIL bloquea la inhibición de la osteoclastogénesis.