Invitrogen™ GLP-1 7-36a Human ELISA Kit

Descripción

The Human GLP-1 7-36a ELISA quantitates Hu GLP-1 7-36a in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GLP-1 7-36a. Principle of the method The Human GLP-1 7-36a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

El glucagón es una hormona peptídica secretada por las células alfa en los islotes de Langerhans pancreáticos. El péptido se sintetiza como un precursor de 180 aminoácidos que convierte en glucagón la prohormona convertasa 2 (PC2). La función principal del glucagón es la gluconeogénesis y la glucogenólisis. Estimula la liberación de glucosa mediante la conversión de glucógeno en el hígado, y también mantiene la homeostasis de la glucosa junto con la insulina.