Invitrogen™ GDNF Human ELISA Kit

Descripción

The Human Glial-Derived Neurotrophic Factor (Hu GDNF) ELISA quantitates Hu GDNF in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu GDNF. Principle of the method The Human GDNF solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

La GDNF es una molécula de homodímero glicosilada de enlace disulfuro. En las primeras citas se describe como un potente factor de supervivencia para las neuronas dopaminérgicas mesencefálicas y posteriormente se demostró que recuperaba estas neuronas en modelos animales de la enfermedad de Parkinson. La GDNF es un factor de supervivencia casi 100 veces más eficiente para las neuronas motoras espinales que para las neurotrofinas. La GDNF es un factor neurotrófico que mejora la supervivencia y la diferenciación morfológica de las neuronas dopaminérgicas y aumenta su absorción de dopamina de alta afinidad.