Invitrogen™ Galectin 3 Mouse ELISA Kit

Descripción

The Mouse Galectin-3 (LGALS3) ELISA quantitates Ms Galectin-3 in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Galectin-3. Principle of the method The Mouse Galectin-3 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Lectina específica de galactosa que une la IgE. Puede mediar con la integrina alfa-3, beta-1 la estimulación por CSPG4 de la migración de las células endoteliales. Junto con el DMBT1, se necesita para la diferenciación terminal de las células epiteliales columnares durante la embriogénesis temprana. En el núcleo: actúa como un factor de empalme pre-ARNm. Participa en respuestas inflamatorias agudas, como la activación y adhesión de neutrófilos, la quimioatracción de macrófagos monocitos, la opsonización de neutrófilos apoptóticos y la activación de mastocitos.