Invitrogen™ Ferric Antioxidant Status Detection Kit

Description

The Ferric Antioxidant Status Detection research-use-only kit is a colorimetric assay designed for the quantification and detection of Ferric Antioxidant Status (also referred as Ferric Reducing Antioxidant Power, FRAP) in serum, plasma, urine, teas, fruit juices, beer, cider, cell lysates, herbal and fruit extracts.

This complete, ready-to-use kit includes clear 96-well plate(s), ferrous chloride standard, ascorbic acid control, and other components to perform the assay. A 96-well microplate reader capable of reading optical density at 560 nm is required for use of this kit.

Performance characteristics
• Assay type: colorimetric detection kit
• Sample types: serum, plasma, urine, teas, fruit juices, beer, cider, cell lysates, herbal and fruit extracts
• Sensitivity: 8.06 μM
• Standard curve range: 31.2–1000 μM
• Reactivity: species independent

Background
Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism. These free radicals (FR) are usually removed or converted into other products in vivo by an array of antioxidants. Organisms have developed complex antioxidant systems to protect themselves from oxidative stress, including the activity of specific enzymes (especially superoxide dismutase, glutathione peroxidase, catalase, glutathione reductase) as well as nonenzymatic compounds with antioxidant activity, such as beta-tocopherol, L-ascorbic acid, glutathione, coenzyme Q10, flavonoids, and albumin. The assay measures the antioxidant ability from all species.

Assay principle
The Ferric Antioxidant Status Detection kit is designed to quantitatively measure antioxidant status in a variety of samples. A ferrous chloride standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in assay buffer and added to the wells. The FRAP color solution is made by mixing reagent A and B with assay buffer. The FRAP color solution is added to all wells and the plate incubated at room temperature. Antioxidant power in the samples reacts with the FRAP color solution to generate a blue-colored product which is read at 560 nm.