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6x-His Tag Monoclonal Antibody (3D5), HRP, Invitrogen™
Mouse Monoclonal Antibody
Marca: Invitrogen R93125
Detalles adicionales : Peso : 0.00525kg
Descripción
NOTE: R931-25 was previously sold under product #46-0707. When re-ordering, please use #R931-25. This antibody can be used to detect expression of C-terminal His fusion proteins from bacterial, insect, and mammalian cells. This product contains enough material for 25 Western blots. It is functionally tested using 100 ng of an E. coli expressed fusion protein containing a C-terminal polyhistidine (6xHis) epitope. Chemiluminescent detection should be performed using a 1 minute exposure. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM). Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.
6x-His Tag Monoclonal antibody specifically detects 6x-His Tag in Tag samples. It is validated for Western Blot.Especificaciones
| 6x-His Tag | |
| Monoclonal | |
| HRP | |
| 6His tag; 6X His; His Tag; Histidine Tag | |
| C-term 6x His synthetic peptide: -His-His-His-His-His-His-COOH | |
| 50 μL | |
| Primary | |
| Antibody | |
| IgG2b |
| Western Blot | |
| 3D5 | |
| PBS with no preservative | |
| Mouse | |
| purified | |
| RUO | |
| Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. | |
| Liquid | |
| Tag |